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95114-250 - 250 x 20 Micro-liters - Perfecta qPCR ToughMix, Low


95114-250 - 250 x 20 Micro-liters - Perfecta qPCR ToughMix, Low


Product description

PerfeCTa qPCR ToughMix, Low ROX is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. PerfeCTa qPCR ToughMix, Low ROX contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of the AccuVue tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product. A key component of PerfeCTa qPCR ToughMix, Low ROX is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix, Low ROX delivers exceptional performance with either fast or conventional PCR cycling protocols. Real-Time PCR Instrument Compatibility:Applied Biosystems 7500Applied Biosystems 7500 FastStratagene Mx3000PStratagene Mx3005PStratagene Mx4000Applied Biosystems ViiA 7Applied Biosystems QuantStudio 12K Flex Contents:2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, AccuVue blue qPCR dye, and stabilizers Storage conditions:PerfeCTa qPCR ToughMix, Low ROX is stable for 2 years when stored in a constant temperature freezer at -20oC. For convenience, it may be stored unfrozen at 2 to 8oC for up to 6 months. After thawing, mix thoroughly before using. Repeated freezin

95114-250 - 250 x 20 Micro-liters - Perfecta qPCR ToughMix, Low

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