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" G-Biosciences' cross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulfhydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules. Functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls, carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents. G-Biosciences' cross-linking agents are used for the following applications: protein structural and functional studies, cell membrane structural studies, protein amp;amp -receptor interactions, cell surface studies, solid-phase immobilization, preparation of immunogens, immunotoxins and other conjugated protein reagents, protein to DNA/RNA cross-linking, reactive group transfer, protein-protein conjugation, and for immunological tools. To select a cross-linking reagent several factors need to be considered (see selection guide). These factors include: Reactive Toward: determines the target residues to be cross-linked (select a reagent that does not interfere with protein function) -Membrane Permeability: for cell surface labeling, select non-membrane permeable reagents -Cleavable: for easy release of cross-linked proteins from solid supports or for further downstream applications -Reversible: an alternative to cleavable reagents -Steric Hinderance: bulky groups around the binding site may require reagents with longer spacer arms. G-Biosciences offers a variety of cross-linking agents, reaction specific buffers and accessories for performing and facilitating cross-linking applications. G-Biosciences' cross-linking agents are divided into groups dependent on the number and similarity of the reactive groups. Photoreactive groups are used when no specific groups are available to react with as photoreactive groups react non-specifically upon exposure to UV lig

ANB-NOS

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The 21st Century Therapist is able to…

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Dedicated to the Development of the 21st Century Therapist

 

 

The 21st Century Therapist is able to…

  • integrate a depth of knowledge, across a number of disciplines, embracing a broad understanding of theories and techniques.
  • craft responsive and personalized therapy for each client.
  • stem resistance and burnout by adopting a dynamic curiosity approach.

 

 

 

 

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Today we talk to Dr Francis Lee Stevens who's latest book, Affective Neuroscience in Psychotherapy, offers an alternative model of psychotherapy, using the patient’s emotions as the focal point of treatment. This unique text approaches emotions as the primary source...

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Making a difference in the Life of a Child:The Relationship is Key Michele Coleman What’s the Problem?Have you ever had a child in your home that resisted doing anything you asked? Did he yell at you? Did she hit her brothers and sisters? Did he hit his peers? Did she...
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The pleasure of curating this magazine never wanes. There is always fascinating material coming in from around the globe. In this vein, we continue our series of international Last Words with Flavio Cannistra from Italy. He begins with a famous quote which produces...
What is time? Physicists say it is the procession of events or experiences that produce an increase in complexity that makes sense to our conscious mind. Over time, we develop a “self” to whom those events and experiences happen and allows us to realize that there are...
Editor’s Note: It is always a thrill when one of our authors receives recognition for excellence. Terry Marks-Tarlow and colleagues, Harris l. Friedman, Yakov Shapiro, & Katthe P. Wolf, have had their book, Fractal Epistemology for a Scientific Psychology...
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A team of researchers from SAHMRI and Flinders University has found a link between the type of microbiome that repopulates the gut following antibiotics and shortened lifespan in mice. The study recently published in Cell Reports, is the first of its kind to examine...

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