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BS-13 - Hook Biotin-PDA Kit - Hook Sulfhydryl Reactive Biotin Re


BS-13 - Hook Biotin-PDA Kit - Hook Sulfhydryl Reactive Biotin Re


Product description

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the selection of the biotinylation reagent itself. G-Biosciences offers a wide range of biotin reagents with variations in their reactive groups, spacer arm lengths, solubility, membrane permeability and reversibility. All of these factors must be considered and are dependent on your protein/peptide. G-Biosciences' HOOK Sulfhydryl Reactive Biotin Reagents react with protein-free sulhydryl groups. These sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl residues are not available, they can be generated by the reduction of disulfides (-S-S-) with reducing agents such as mercaptoethylamine, or by modifying lysine epsilon-amines with Traut's reagent or SATA. After reduction, excess reducing agent must be removed before coupling. In addition, a metal chelating agent (EDTA), an anti-oxidant, should be used to reduce the chances of re-oxidation of sulfhydryls to disulfides. There are three different reactions employed to couple biotin reagents to sulfhydryl residues and involve either iodoacetyl, maleimide, or pyridylthiol groups. G-Biosciences' HOOK Sulfhydryl Reactive Biotin Reagents are each available as the reagent alone or as part of a biotin labeling kit. The labeling kits are designed and supplied with all the necessary reagents, equipment and instructions necessary for optimal reaction conditions, efficient labeling, removal of unbound biotin, and quantification of biotin labeling, for the conjugation of biotin to protein primary sulfhydryl groups. Each kit is supplied with 25 milligrams of the specifi

BS-13 - Hook Biotin-PDA Kit - Hook Sulfhydryl Reactive Biotin Re

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